Wednesday, March 12, 2014

Relation of the inferior alveolar nerve and vessels ..consideration for dental implants in the posterior mandible


From Jay Matani and Mohit Kheur



The anatomic inter relationship of the neurovascular structures within the inferior alveolar canal: A Cadaveric and Histological study.
Abstract
Objective: The location and inter relationship of the structures of the inferior alveolar neurovascular bundle within the mandibular canal has not been clearly defined. The knowledge of the same is important while planning surgeries in the posterior mandible
Methods: 8 cadaveric mandibles were dissected and sections were made at the distal aspect of every tooth. The inferior alveolar neurovascular bundle was identified and examined for the location of the inferior alveolar artery, vein and nerve. Hematoxylin and Eosin sections were made for each specimen to confirm the position of these structures.
Results: All the sections in all the specimens confirmed that a blood vessel lies superior to the nerve. This position appeared consistent in all the positions relative to all the posterior teeth. There was a variation in the bucco-lingual positioning of these structures relative to each other for the various mandibles.
Conclusion: A blood vessel is found to always lie superior to the inferior alveolar nerve within the mandibular canal. Variations in the inter relationship of the structures is present.
Significance: This cadaveric study proves that all along the course of the neurovascular bundle, at various cross sections studied, the inferior nerve is always inferior to a blood vessel. There can be great variations to the positioning of the structures within the neurovascular in the bucco-lingual dimension and also in the exit of the nerve in various mandibles. Knowledge of the location of the structures is of importance during surgical procedures carried out in the vicinity of these structures.
Keywords: mandible; alveolar nerve, inferior; anatomy; dental implants.

Introduction:
The knowledge of the anatomical position of the structures within the inferior alveolar canal is important for any surgical procedure in the posterior mandible. The inferior alveolar neurovascular bundle enters the mandible at the mandibular foramen and runs downward and medial within the mandibular canal (MC) to exist from the mental foramen. The neurovascular bundle that occupies the inferior alveolar canal contains the inferior alveolar nerve, the inferior alveolar artery and the inferior alveolar vein.
Osteotomies for endosseous implants should not be made until the position of the mandibular canal is established. Prior to any surgical procedure, radiographic examination is carried out either using panoramic radiographs or conventional or computerized tomographies. Peker reported that the mandibular canal could not be located in 19.4 % of the panoramic radiographs and 13.9% of the conventional tomographs [1]. During various surgical procedures of the posterior mandible there is a risk of complications involving the inferior alveolar neurovascular bundle. These could include altered sensation, numbness and pain, complete loss of sensation or excessive bleeding. Inferior alveolar nerve (IAN) injury occurs as a complication of mandibular third molar surgery with a frequency varying from 0.5% to 8% [2, 3].
Encountering anatomic variations is possible during surgical procedures and such incidences can manifest from ineffective mandibular blocks to major complications after surgery. Kilic examined the mandibular canal in hemimandibles of cadavers and reported the presence of several branches of the inferior alveolar nerve at different sections [4]. Pogrel examined cadaveric mandibles for understanding the relationship of the neurovascular bundle in the 3rd molar region [5]. He reported that there was a consistent presence of the vein along with the nerve. The artery was solitarily present. The precise location of these structures in relation to each other at different location in the mandibular canal has not been reported. This knowledge may be of some importance in procedures involving third molars and implant-related procedures.
The aim of this study is to understand the intracanal relationship of these structures during the course traversed by the neurovascular bundle from the 3rd molar up to its exit from the body of the mandible.

Materials and methods:
Eight fully dentate hemi mandibles were selected from preserved human cadavers. Following gross dissection, the mandibles were carefully separated from all muscle attachments keeping the inferior alveolar neurovascular bundle intact at the mandibular and mental foramen region. A water cooled carborundum disc was used to make vertical sections distal to each tooth. The neurovascular bundle was exposed and the vein, artery, and nerve were visually identified to determine their exact orientation within the canal and in the mandible (Figure 1a to 1f). The gross identification of the structures was done under 6x magnification.


A histological evaluation was done for all the sections to identify and confirm the location of the artery, vein and nerve to each other (Figure 2). The sectioned specimens were fixed for 24 hours in 10% neutral buffered formalin and then kept in decalcifying agent (20% EDTA) for 15 days. The specimens were then immersed in a combination of 5 % formic and 10 % nitric acid for a period of 15 days.  After decalcification was complete, the mandibles were processed and embedded in paraffin and sectioned into 5-µm slices. The sections were stained with routine Hematoxylin and Eosin stain and then studied under a compound microscope for further evaluation. The sections were taken at 4x (objective) magnification scanner view.



Results:
The mandibular canal and neurovascular bundle of the mandibular canal were visible in all hemimandibles. The relationship of the artery, vein and nerve to each was examined from the external cortices of the mandible, from distal of the 3rd molar until its exit from the mental foramen. A schematic representation of the structures was made to understand their relationship to each other (Figure 3). In all the cross sections studied a blood vessel was evident superior to the position of the nerve. Out of the 25 cross sections studied, a vein was present superior to the nerve in 19 (76%) of the sections. Both the blood vessels were present superior to the nerve in 16 (64%) out of the 25 cross sections. The bucco-lingual relationship of the structures had variation. In only 12 out of 25 sections (48%) one blood vessel was present buccal to the inferior alveolar nerve.



Discussion:
The anatomy of inferior alveolar canal has been documented in various textbooks and previous cadaveric studies. However the relationship of the structures within the canal is still unclear. The relative location of the inferior alveolar canal and associated foramina in adults remain fairly constant without regard to age and sex [6]. Levine reported that the bucco-lingual IAN canal position varied with the age and race of the patient [7]. Many variations have been reported in the literature with regards to the inferior alveolar nerve and it course. Lui classified the course of the IAN as linear curve, spoon-shape curve, elliptic-arc curve and turning curve [8]. These variations were seen on the pantomographs. Juodzbalys reported that the mandibular canal bifurcates in the inferior superior or medial lateral plane [9]. Observation of the MC shape from the mandibular foramen towards the anterior part of the mandible showed several characteristic variations in the form of round/oval, tear-drop and dumbbell shape [10]. The identification of these variations may not be possible without conventional or computerized tomography. Therefore there is a need to understand the relationship of the structures within the canal.
Pogrel reported the relationship of these structures in the third molar region and concluded the presence of the vein superior to the nerve [5]. The artery was found to be lingual to the nerve. This concurs with the results obtained from this cadaveric study. However his study reports the locations in the 3rdmolar regions only, which are not significant for dental implant surgery. When the roof of the inferior alveolar canal is breached bleeding may occur and this may be due to the damage to the vein. However if the insertion of the implant is halted at this point, damage to the nerve can be avoided.
Conclusion:
1.    Variations were seen in the intra canal position of the inferior alveolar nerve, artery and vein in different regions of the same mandible and in different mandibles.
2.    A blood vessel was found to always lie superior to the inferior alveolar nerve within the mandibular canal.
3.    Variations are seen in the bucco-lingual positions of the structures within the mandibular canal in different regions of the same mandible and in different mandibles.












References:
1.    Peker I, Alkurt MT, Mihcioglu T. The use of 3 different imaging methods for the localization of the mandibular canal in dental implant planning. The International Journal of Oral & Maxillofacial Implants 2008; 23: 463-70.
2.    Blaeser BF, August MA, Donoff RB, et al: Panoramic radiographic risk factors for inferior alveolar nerve injury after third molar extraction. Journal of Oral and Maxillofacial Surgery 2003; 61:417- 21.
3.    Nakagawa Y, Ishii H, Nomura Y, et al: Third molar position: Reliability of panoramic radiography. Journal of Oral and Maxillofacial Surgery 2007; 65:1303- 8.
4.    Kilic C, Kamburoglu K, Ozen T, Balcioglu HA, Kurt B, Kutoglu T, et al. The Position of the Mandibular Canal and Histologic Feature of the Inferior Alveolar Nerve. Clinical Anatomy. 2010; 23:34–42.
5.    Pogrel MA, Dorfman D and Fallah H. The Anatomic Structure of the Inferior Alveolar Neurovascular Bundle in the Third Molar Region. Journal of Oral and Maxillofacial Surgery 2009; 67:2452-2454.
6.    Angel JS, Mincer HH, Chaudhry J and Scarbecz M. Cone-beam Computed Tomography for Analyzing Variations in Inferior Alveolar Canal Location in Adults in Relation to Age and Sex. Journal of Forensic Sciences, 2011; 56: 216-19.
7.    Levine MH, Goddard AL and Dodson TB. Inferior Alveolar Nerve Canal Position: A Clinical and Radiographic Study. Journal of Oral and Maxillofacial Surgery 2007; 65:470-474.
8.    Liu T, Xia B, Gu Z. Inferior alveolar canal course: a radiographic study. Clinical Oral Implants Research. 2009; 20: 1212–1218.
9.    Juodzbalys G, Wang HL, Sabalys G. Anatomy of Mandibular Vital Structures. Part I: Mandibular Canal and Inferior Alveolar Neurovascular Bundle in relation with Dental Implantology. Journal of Oral & Maxillofacial Research 2010 1(1):e2.
10. Megumi Ueda, Kenji Nakamori, Kaori Shiratori, Tomohiro Igarashi, Takanori Sasaki, Naoki Anbo, et al. Clinical Significance of Computed Tomographic Assessment and Anatomic Features of the Inferior Alveolar Canal as Risk Factors for Injury of the Inferior Alveolar Nerve at Third Molar Surgery. Journal of Oral and Maxillofacial Surgery 2012; 70:514-520.














Legends:
Figure 1a to 1f: Cross-sections of cadaveric mandibles
Figure 2: Histologic examination of the specimen showing Inferior alveolar nerve, artery and vein
Figure 3: Schematic representation of the neurovascular bundle







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